Development and validation of an LC-MS/MS method for pharmacokinetic study of lobetyolin in rats

A simple and selective liquid chromatography tandem with mass spectrometry (LC-MS/ MS) method for quantification of lobetyolin in rat plasma was developed validated. Chromatographic separation achieved on a Thermo ODS C18 reversed-phase column using 0.1% aqueous formic acid-methanol (50:50, v/v) an isocratic elution mode at flow rate 0.4 mL.min-1. LC/MS performance done positive ion ESI the MS/MS transitions were monitored m/z 419.3 [M+Na]+ → 203.1 394.9 231.9 IS, respectively. The assay exhibited linear dynamic range over 1.0-500 ng.mL-1 plasma. Both precision (%RSD) accuracy (RE%) within acceptable criteria (<15%). Recoveries ranged from 87.0% to 95.6%, matrix effects 91.0% 101.3%. After oral administration, peak concentration obtained as 60.1 1.0 h. proposed LC-MS/MS could be applied pharmacokinetic study employing 66 samples 6 Wistar rats.